SeaKem® GTG® Agarose
For separation and manipulation of DNA > 1,000 bp.

Introduction

SeaKem GTG agarose is a standard gelling
temperature, high gel strength agarose for
resolving DNA fragments between 100-23,000
bp. This agarose is specifically designed for
preparative DNA electrophoresis. A GTG (Genetic
Technology Gradeô) agarose, it is extensively
performance tested to ensure compatibility with
routine molecular biology techniques.
Analytical Specifications
Gelling temperature (1.5%) 36 ± 1.5° C
Melting temperature (1.5%) ≥90° C
Gel strength (1%) ≥1,200 g/cm²
Applications
• Analytical electrophoresis of DNA and RNA
> 1,000 bp
• Blotting of DNA and RNA
Suggested Agarose Concentrations
Size Range Final Agarose Concentration (%)
(Base Pairs) 1X TAE Buffer 1X TBE Buffer
1000-23,000 0.60 0.50
800-10,000 0.80 0.70
400-8,000 1.00 0.85
300-7,000 1.20 1.00
200-4,000 1.50 1.25
100-3,000 2.00 1.75
Dye Mobility Table
Migration of double-stranded DNA in relation to
Bromophenol Blue (BPB) and Xylene Cyanol (XC)
in SeaKem GTG agarose gels.
1X TAE Buffer % 1X TBE Buffer
XC BPB Agarose XC BPB
24,800 2,900 0.30 19,400 2,850
11,000 1,650 0.50 12,000 1,350
10,200 1,000 0.75 9,200 720
6,100 500 1.00 4,100 400
3,560 370 1.25 2,500 260
2,800 300 1.50 1,800 200
1,800 200 1.75 1,100 110
1,300 150 2.00 850 70
Precautions
Always wear eye protection when dissolving
agarose and guard yourself and others against
scalding solutions.
Microwave Instructions for Agarose
Preparation
1. Choose a beaker that is 2-4 times the
volume of the solution.
2. Add room temperature 1X or 0.5X
electrophoresis buffer and a stir bar to the
beaker.
3. Slowly sprinkle in the agarose powder
while the solution is rapidly stirred.
4. Remove the stir bar if not Teflon®
coated.
5. Weigh the beaker and solution before
heating.
6. Cover the beaker with plastic wrap.
7. Pierce a small hole in the plastic wrap for
ventilation.
8. Heat the beaker in the microwave oven on
High power until bubbles appear.
9. Remove the beaker from the microwave
oven.
10. Caution: Any microwaved solution
may become superheated and foam
over when agitated.
11. GENTLY swirl the beaker to resuspend
any settled powder and gel pieces.
12. Reheat the beaker on HIGH power until
the solution comes to a boil.
13. Hold at boiling point for 1 minute or
until all of the particles are dissolved.
14. Remove the beaker from the microwave
oven.
15. GENTLY swirl the beaker to thoroughly
mix the agarose solution. After
dissolution, add sufficient hot distilled
water to obtain the initial weight.
16. Mix thoroughly.
17. Cool the solution to 50-60° C prior to
casting.
Hot Plate Instructions for Agarose
Preparation
1. Choose a beaker that is 2-4 times the
volume of the Solution.
2. Add room-temperature electrophoresis
buffer and a stir bar to the beaker.
3. Slowly sprinkle the agarose powder while
the solution is rapidly stirred.
4. Weigh the beaker and solution before
heating.
5. Cover the beaker with plastic wrap.
6. Pierce a small hole in the plastic wrap for
ventilation.
7. Bring the solution to a boil while stirring.
8. Maintain gentle boiling until all the
agarose is dissolved (approximately 10
minutes).
9. Add sufficient hot distilled water to obtain
the initial weight.
10. Mix thoroughly.
11. Cool the solution to 50-60° C prior to
casting.
Ordering Information:
Catalog No. Size
50071 25 g
50070 125 g
50074 500 g
These products may be used as in vitro medical devices in
accordance with the U.S. Food, Drug, and Cosmetics Act.